The adenosine triphosphatase from the moderate halophile, Vibrio costicola
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چکیده
منابع مشابه
Role of membrane-bound 5'-nucleotidase in nucleotide uptake by the moderate halophile Vibrio costicola.
Intact cells of Vibrio costicola hydrolyzed ATP, ADP, and AMP. The membrane-bound 5'-nucleotidase (C. Bengis-Garber and D. J. Kushner, J. Bacteriol. 146:24-32, 1981) was solely responsible for these activities, as shown by experiments with anti-5'-nucleotidase serum and with the ATP analog, adenosine 5'-(beta gamma-imido)-diphosphate. Fresh cell suspensions rapidly accumulated 8-14C-labeled ade...
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Phosphoenolpyruvate carboxykinase (PEPCK) was purified to homogeneity from the moderately halophilic bacterium Vibrio costicola. The enzyme is monomeric, with an Mr of 62,000, as determined by the Svedberg equation, by using values of s0(20,w) 4.4 x 10(-13) s, D20,w 6.13 x 10(-7) cm2.s-1 and v 0.719 cm3.g-1. Compared with other, non-halophilic, PEPCKs, the enzyme from V. costicola had a signifi...
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In vitro protein synthesis was studied in extracts of the moderate halophile Vibrio costicola by using as mRNAs the endogenous mRNA of V. costicola and the RNA of the R17 bacteriophage of Escherichia coli. Protein synthesis (amino acid incorporation) was dependent on the messenger, ribosomes, soluble cytoplasmic factors, energy source, and tRNA(FMet) (in the R17 RNA system) and was inhibited by...
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The membrane adenosine triphosphatase from SfrepfoCOCCUS faecalis has been purified by heat treatment, gel filtration through Agarose, and repeated chromatography on diethylaminoethyl cellulose. The purified ATPase appears to be homogeneous as judged from gel electrophoresis and sedimentation velocity studies. Sedimentation equilibrium studies, which are presented in the succeeding paper, also ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1978
ISSN: 0378-1097
DOI: 10.1016/0378-1097(78)90079-4